Cellulolytic fungi isolated by the screened substrate method
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چکیده
منابع مشابه
Isolation and Screening of Cellulolytic Fungi
A large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing cellulose. Fungi are the main cellulase producing microorganisms. In this purposed study, four fungal species belonging to two genera i.e. Trichoderma and Aspergillus were isolated from different sources, screened and...
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This study focused on the evaluation of cellulolytic and hemicellulolytic fungi isolated from sawdust compost (SDC) and coffee residue compost (CRC). To identify fungal isolates, the ITS region of fungal rRNA was amplified and sequenced. To evaluate enzyme production, isolates were inoculated onto wheat bran agar plates, and enzymes were extracted and tested for cellulase, xylanase, β-glucanase...
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Atkinson, B. & Green, A. L. (1957). Tran. Faraday Soc. 53, 1334. Balls, A. K. & Jansen, E. F. (1952). Advane. Enzymol. 13, 321. Childs, A. F., Davies, D. R., Green, A. L. & Rutland, J. P. (1955). Brit. J. Pharmacol. 10, 462. Cohen, J. A., Oosterbaan, R. A., Warringa, M. G. P. J. & Jansz, H. S. (1955). Disc. Faraday Soc. 20, 114. Cunningham, L. W. (1954). J. biol. Chem. 207, 443. Cunningham, L. ...
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Cellulolytic fungi can easily be screened within 2 d for the production of cellulolytic enzymes by staining with Congo red, or by measuring the amount of reducing sugar (glucose) produced with the dinitrosalicyclic acid reagent method. Endoglucanase activity is visible in carboxymethyl cellulose agar plates after staining with Congo red, and fixing with HCI or NaOH. This method is essentially b...
متن کاملSimple dye release assay for determining cellulolytic activity of fungi.
A colorimetric assay, based on the release of dye from a paper substrate, is described as a simple and rapid means of detecting cellulolytic activity. It is readily adaptable to rapidly screening in situ large numbers of fungal liquid cultures. Since it is a nondestructive assay, samples can be recovered for later use.
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ژورنال
عنوان ژورنال: Experientia
سال: 1968
ISSN: 0014-4754,1420-9071
DOI: 10.1007/bf02138354